We measured resolution acuity separately for gratings with positive (+S) and negative (−S) S-cone contrast at different eccentricities in the temporal retina to determine if the resolution performance is limited by a single bipolar opponent mechanism. Gratings were modulated from an achromatic background in either the 90 or 270 deg direction in DKL space. Two additional directions were tested to examine the effects of increased lens yellowing and macular pigment absence in the periphery. The gratings used slow temporally rising hue (co-sinusoidal step) to aid selective S-cone ON or OFF stimulation. Resolution acuity was measured using different luminance ratios of the grating bars with the background around the equiluminant point. The data displayed a plateau where the acuity was minimal and mediated by the sparse S-cone pathway, with observer performance rising on either side. The acuity at the minimum was consistently higher for +S gratings than −S gratings beyond 10 deg eccentricity. Above the resolution limit, an aliased pattern was detected for both types of gratings, indicating that the resolution acuity was limited by the density of postreceptoral neurons. The results suggest that the resolution of gratings with opposite S-cone contrast polarity is limited by separate and rectified mechanisms of different distribution.